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protean isoelectric focusing (ief) tray  (Bio-Rad)


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    Structured Review

    Bio-Rad protean isoelectric focusing (ief) tray
    Protean Isoelectric Focusing (Ief) Tray, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protean+isoelectric+focusing+(ief)+tray/Focusing+Tray+With+Lid/pmc06912385-117-10-12
    Average 90 stars, based on 1 article reviews
    protean isoelectric focusing (ief) tray - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Electrofocusing:

    Article Title: Drosophila NUAK functions with Starvin/BAG3 in autophagic protein turnover.
    Article Snippet: .. The samples were transferred to separate lanes of a BioRad Protean IEF focusing tray along with BioRad ReadyStrip IPG strips, pH 5–8, 7cm (BioRad Laboratories, Hercules, CA) and covered with mineral oil. .. Using a BioRad Protean IEF cell, the strips were passively rehydrated (20 ̊C for 12 hrs) and then focused (4000V, Rapid, 15000 Vhrs).

    Article Title: Iron-Induced Changes in the Proteome of Trichomonas vaginalis Hydrogenosomes
    Article Snippet: .. Combined iTRAQ-labeled samples were dissolved in 250 μl of 2 M urea and poured into the 17 cm focusing tray of a Protean IEF Cell (Bio-Rad, Hercules, CA, USA). .. The sample was covered with 17 cm IPG strips (pH 3–10, Bio-Rad) without paper wicks or oil.

    Article Title: Site-specific phosphorylation and caspase cleavage of GFAP are new markers of Alexander disease severity
    Article Snippet: Immobilized pH gradient (IPG) strips (Biorad; 11 cm; pH 4–7; 1632015) were passively rehydrated in 2-D starter kit rehydration/sample buffer overnight. .. Cup loading method was employed to load the protein samples in cathode side (as isoelectric point of GFAP is 5.2) of the Protean IEF cell tray (Biorad; 1654020). ..

    Article Title: BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
    Article Snippet: .. The strips were transferred to a Protean IEF (BIO-RAD) focusing tray and laid gel side down across wet paper wick-covered electrodes and covered with mineral oil. .. The strips were focused in a Bio-Rad Protean IEF Cell at 20 °C, rapid ramp 0–250 V, 15 min; rapid ramp 250–8000 V 2 h; hold 8000 V to a total of 35,000 Vh.

    Article Title: Comparative brain proteome analysis of an Indole Alkaloid of Kratom, Mitragynine and Kratom juice in rats.
    Article Snippet: Mitragyna speciosa, commonly known as kratom, is a medicinal plant that is widely used for various medical conditions.. The major alkaloid in kratom is mitragynine which binds partially to opioid receptors to produce opioid-like effects.. In this study, we compared the effects of mitragynine various doses with kratom juice on neurobehavioral, neurochemical changes as well as calretinin protein expression.

    Article Title: Histological and proteome analyses of Microbacterium foliorum -mediated decrease in arsenic toxicity in Melastoma malabathricum .
    Article Snippet: Subsequently, the electrode tray was covered and put into the PROTEAN IEF Cell (Bio-Rad, USA) and the IEF method was specified. .. The electrode tray was removed from the PROTEAN IEF Cell (Bio-Rad) after completion of the IEF, and the strips were transferred upside down to a strip holder gel. .. SDS equilibration buffer (6 M urea, 75 mM pH 8.8 Tris HCl, 29.3% (v/v) glycerol, 2% (w/v) SDS, 0.02% (w/v) bromophenol blue) with DTT (100 mg/10 mL) was first added, then SDS 3 Biotech (2021) 11:336 1 3 336 Page 4 of 17 equilibration buffer with iodoacetamide (250 mg/10 mL) was used and the strip holder was shaked for 15 min on a gyro-rocker.

    Article Title: Histological and proteome analyses of Microbacterium foliorum -mediated decrease in arsenic toxicity in Melastoma malabathricum .
    Article Snippet: .. Subsequently, the electrode tray was covered and put into the PROTEAN IEF Cell (Bio-Rad, USA) and the IEF method was specified. .. The electrode tray was removed from the PROTEAN IEF Cell (Bio-Rad) after completion of the IEF, and the strips were transferred upside down to a strip holder gel.

    Multiplex sample analysis:

    Article Title: Iron-Induced Changes in the Proteome of Trichomonas vaginalis Hydrogenosomes
    Article Snippet: .. Combined iTRAQ-labeled samples were dissolved in 250 μl of 2 M urea and poured into the 17 cm focusing tray of a Protean IEF Cell (Bio-Rad, Hercules, CA, USA). .. The sample was covered with 17 cm IPG strips (pH 3–10, Bio-Rad) without paper wicks or oil.

    other:

    Article Title: Oligomeric States and Hydrodynamic Properties of Lysyl Oxidase-Like 2
    Article Snippet: The IPG strips were transferred to the running tray of a PROTEAN ® i12TM IEF system (Bio-Rad Laboratories) before being overlaid with another 5 mL of mineral oil and run using the recommended procedure for 11 cm pH 3–10 IPG strips (e.g., 250 volts for 20 min, 8000 volts for 1 h, and 8000 volts for 26,000 volt hours, with a total run time of approximately 6 h at 20 °C).

    Stripping Membranes:

    Article Title: Histological and proteome analyses of Microbacterium foliorum -mediated decrease in arsenic toxicity in Melastoma malabathricum .
    Article Snippet: Subsequently, the electrode tray was covered and put into the PROTEAN IEF Cell (Bio-Rad, USA) and the IEF method was specified. .. The electrode tray was removed from the PROTEAN IEF Cell (Bio-Rad) after completion of the IEF, and the strips were transferred upside down to a strip holder gel. .. SDS equilibration buffer (6 M urea, 75 mM pH 8.8 Tris HCl, 29.3% (v/v) glycerol, 2% (w/v) SDS, 0.02% (w/v) bromophenol blue) with DTT (100 mg/10 mL) was first added, then SDS 3 Biotech (2021) 11:336 1 3 336 Page 4 of 17 equilibration buffer with iodoacetamide (250 mg/10 mL) was used and the strip holder was shaked for 15 min on a gyro-rocker.



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    90
    Bio-Rad protean isoelectric focusing (ief) tray
    Protean Isoelectric Focusing (Ief) Tray, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protean+isoelectric+focusing+(ief)+tray/Focusing+Tray+With+Lid/pmc06912385-117-10-12
    Average 90 stars, based on 1 article reviews
    protean isoelectric focusing (ief) tray - by Bioz Stars, 2026-10
    90/100 stars
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